Indranil Dasgupta

Friday Seminar by Dr. Nidhi Singh


GKP Lab/ Friday/ September 13, 2019/ 3.30 pm/ How nucleotide-binding site leucine-rich repeat (NLR) receptors trigger immune transcriptional activation: The Rice pigm Locus story.
Category: Research
Posted by: bedineel

How nucleotide-binding site leucine-rich repeat (NLR) receptors trigger immune transcriptional activation: The Rice Pigm Locus Story.

                                                   

Crop breeding aims to balance disease resistance with yield; however, single resistance (R) genes can lead to resistance breakdown. The rice Pigm locus contains a cluster of genes encoding nucleotide-binding leucine-rich repeat (NLR) receptors that confer durable resistance to the fungus Magnaporthe oryzae without yield penalty. Among these NLR receptors, PigmR confers durable resistance, whereas PigmS competitively attenuates PigmR homodimerization to suppress resistance. PigmS increases seed production to counteract the yield cost induced by PigmR. PIBP1 (PigmR-INTERACTING and BLAST RESISTANCE PROTEIN 1), an RRM (RNA recognition motif) protein that specifically interacts with PigmR and other similar NLRs to trigger blast resistance. PigmR-promoted nuclear accumulation of PIBP1 ensures full blast resistance. PIBP1 is found to bind with DNA and function as unconventional transcription factors at the promoters of the defense genes OsWAK14 activating their expression. Knockout of PIBP1 greatly attenuated blast resistance. Collectively, these studies revealed previously unexplained RRM transcription factors that directly interact with NLRs to activate plant defense.

 

Suggested Readings:

 

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2-    N. Zhang et al., IMA Fungus 7, 155–159 (2016).

 

3-    T. Maekawa et al., Cell Host Microbe 9, 187–199 (2011).

 

4-    M. Bernoux et al., Cell Host Microbe 9, 200–211 (2011).

 

5-    Keran Zhai, Yiwen Deng et al., Molecular Cell 74, 996–1009 (2019).

 

6-    Yiwen Deng, Keran Zhai et al., Science 355, 962–965 (2017).